色中色

色中色 added a powerful new tool to the campus鈥檚 Imaging and Microscopy Facility. The newly acquired Zeiss LSM 880 is a state-of-the-art laser scanning confocal microscope that represents a huge leap forward for the university鈥檚 imaging capabilities.

鈥淲ithin 色中色, this is by far the most advanced, state-of-the-art light microscope,鈥� Professor Anand Subramaniam said.

Subramaniam led the three-person team 鈥� which included School of Natural Science professors and 鈥� that brought the microscope to 色中色. But getting there wasn鈥檛 easy.

First, they had to convince the (ORED) that the new confocal was among the university鈥檚 top research priorities. Subramaniam鈥檚 was one of many proposals ORED was considering. But, according to Vice Chancellor for Research Sam Traina, 鈥淭his was a pretty easy choice.鈥�

鈥淭he proposal written by Professor Subramaniam and his colleagues was clearly identified as the best of the group by a panel of faculty peers at the university,鈥� Traina said.

With ORED鈥檚 endorsement, Subramanian and company next had to convince the National Science Foundation that 色中色 was deserving of a Major Research Instrumentation (MRI) award.

The grant was awarded last September.

So how much does a top-of-the-line confocal microscope cost? A lot, it turns out. The NSF MRI grant covered $311,815, while the , the and ORED covered the remaining $153,228. That鈥檚 $465,043 for one microscope.

And according to Subramaniam and Ghosh, it鈥檚 worth every penny.

Previously, 色中色 researchers seeking access to comparable equipment had to travel to microscopy facilities housed at Stanford or UC Berkeley. More than just inconvenient, in some cases it was impossible. Some samples are too sensitive to travel; others might survive the journey only to yield data unfit for publication.

Bringing the Zeiss LSM 880 to 色中色 solved this. With the same state-of-the-art equipment on campus, researchers can pursue avenues of inquiry they might otherwise have avoided.

But what makes the microscope such a valuable research tool? The team points to its versatility, power and speed.

鈥淚t鈥檚 important to have multimodal imaging capabilities,鈥� Subramaniam explained. 鈥淢ost confocals are optimized for biological science research 鈥� think squishy, wet things 鈥� or materials sciences, with hard things like glass and ceramics. We needed something that could do both鈥�

The confocal has six lasers, allowing for multicolor imaging. It also has the latest Airyscan technology, a feature that minimizes signal loss and brings resolution down to 110 nanometers (for perspective, a human hair is about 100,000 nanometers thick), allowing the microscope to discern details other confocals cannot.

The microscope is also fast. Scientists use confocals because they can capture still images in very quick succession, essentially producing a movie of microscopic processes in action. Typically, there鈥檚 a trade-off between speed and resolution. The more seamless the movie, the blurrier the image. This microscope mitigates that problem, providing high-speed, high-resolution dynamic imaging.

For Subramaniam, this feature is key. His lab develops engineering approaches for synthesizing artificial cells. Understanding the dynamic processes involved in the earliest stages of self-assembly of molecules is crucial. How do a bunch of lipids, essentially individual fat molecules, come together to form structures that resemble hollow cells? This microscope will help him and his students figure it out.

For Ghosh, whose lab is developing high-efficiency photovoltaic devices, she and her students need to know how a device鈥檚 microstructure affects its performance. To do this, Ghosh needs two kinds of microscopes. They already had one. Now they have the other.

The new confocal complements the Zeiss Gemini SEM 500 scanning electron microscope acquired last year. The confocal鈥檚 鈥渟huttle and find鈥� feature bridges the two instruments by allowing users to identify sites of interest within a sample and then transfer the sample to the SEM. The SEM will then automatically probe the same sites, which Ghosh thinks is 鈥渢he coolest capability of this instrument.鈥�

鈥淚t can correlate an optical image from the confocal microscope to structural data from the scanning electron microscope,鈥� Ghosh explained. 鈥淭his will give us unique insights into optimizing and improving photovoltaic devices.鈥�

Subramaniam adds, 鈥淭his bridging capability allows us to further interrogate our samples down to the 1 nanometer scale.鈥�

But Subramaniam and his collaborators aren鈥檛 the only ones excited about the new microscope. As Ghosh put it, 鈥淎 world-class research university must have significant core facilities to support the needs of its researchers.鈥�

This microscope will do just that. At least 20 faculty, along with their graduate students and research staff members, are expected to regularly use the instrument.

鈥淭he confocal is an instrument that will benefit the research enterprise at 色中色,鈥� Subramaniam said. 鈥淲e encourage users who were not in the initial grant to contact us if they wish to incorporate confocal microscopy into their research programs鈥�

Moreover, this microscope makes 色中色 a magnet for top-tier graduate students, postdocs and faculty. It brings the campus鈥檚 imaging facilities on par with long-established research institutes and demonstrates that the university is capable of distinguishing itself when pitted against other Ph.D.-granting institutions competing for the same prestigious grants.

鈥淚t was clear to all of us that acquisition of this instrument would greatly enhance the research and education environment at 色中色,鈥� Traina said.